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제117회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Structure and interaction of Streptococcus pyogenes Cas proteins in Class 2 CRISPR-Cas system

등록일
2016년 2월 11일 14시 30분 47초
접수번호
0398
발표코드
BIO2-2 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 09시 : 25분
발표형식
심포지엄
발표분야
생명화학 - 유전체 교정과 생명화학 I (Genome Editing and Life Chemistry I)
저자 및
공동저자
배의영
서울대학교 농생명공학부, Korea
Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins form a microbial defense system against invading foreign nucleic acids. Cas1 is a universal Cas protein found in all subtypes of CRISPR-Cas systems, and its role is implicated in new spacer acquisition during CRISPR-mediated immunity. Here we report the crystal structure of Streptococcus pyogenes Cas1 (SpCas1) in a Class 2 CRISPR-Cas system and characterize its interaction with S. pyogenes Csn2 (SpCsn2), another Cas protein involved in spacer acquisition. The SpCas1 structure reveals a unique conformational state distinct from its Class 1 homologues, resulting in a disruption of potential metal-binding sites for catalysis. We show that SpCas1 directly interacts with SpCsn2 and identify the binding interface and key residues for the Cas complex formation. These results provide structural information for a Class 2 Cas1 protein, a previously uncharacterized Cas1 homologue and lay a foundation for studying multiprotein Cas complexes functioning in the adaptive immunity of Class 2 CRISPR-Cas systems.

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