abs

학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

현재 가능한 작업은 아래와 같습니다.
  • 01월 01일 09시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

Custom-DNA binding proteins and artificial transcription factors

등록일
2006년 2월 24일 14시 30분 26초
접수번호
1232
발표코드
금16D5심 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 11시 : 30분
발표형식
심포지엄
발표분야
생명화학 - 생명1. Cell Signaling
저자 및
공동저자
김진수
서울대학교 화학부,
We have developed novel methods for generating sequence-specific DNA-binding proteins that recognize predetermined DNA sequence elements. These DNA-binding proteins were then fused to domains that rendered the proteins capable of either up- or down-regulating the expression of genes whose promoter regions contain the target DNA sequences. To generate these so-called “designer” transcription factors, we first developed a modified one-hybrid selection method in yeast to isolate zinc finger domains with diverse DNA-binding specificities. We extensively screened zinc finger domains derived from sequences in the human genome and isolated 56 zinc fingers with distinct DNA-binding specificities. We used these zinc fingers as modular building blocks in the construct of novel, sequence-specific DNA-binding proteins. Fusion of these novel zinc finger proteins with either a transcriptional activation or repression domain yielded potent transcriptional activators or repressors, respectively. Our approach, termed GeneGrip technology, should facilitate the construction of customized DNA-binding proteins for use in research, medicine, and biotechnology.

상단으로