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Custom-DNA binding proteins and artificial transcription factors

Submission Date :
2 / 24 / 2006 , 14 : 30 : 26
Abstract Number :
97022412326
Presenting Type:
Symposium
Presenting Area :
생명화학 - 생명1. Cell Signaling
Authors :
김진수
서울대학교 화학부,
Assigned Code :
금16D5심 Assigend Code Guideline
Presenting Time :
금 11시 : 30분
We have developed novel methods for generating sequence-specific DNA-binding proteins that recognize predetermined DNA sequence elements. These DNA-binding proteins were then fused to domains that rendered the proteins capable of either up- or down-regulating the expression of genes whose promoter regions contain the target DNA sequences. To generate these so-called “designer” transcription factors, we first developed a modified one-hybrid selection method in yeast to isolate zinc finger domains with diverse DNA-binding specificities. We extensively screened zinc finger domains derived from sequences in the human genome and isolated 56 zinc fingers with distinct DNA-binding specificities. We used these zinc fingers as modular building blocks in the construct of novel, sequence-specific DNA-binding proteins. Fusion of these novel zinc finger proteins with either a transcriptional activation or repression domain yielded potent transcriptional activators or repressors, respectively. Our approach, termed GeneGrip technology, should facilitate the construction of customized DNA-binding proteins for use in research, medicine, and biotechnology.