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  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제117회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Real-time observation of the neurotransmitter release process by single-molecule FRET

2016년 2월 18일 16시 13분 17초
PHYS.O-9 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 10시 : 40분
물리화학 - 일반 구두발표 (General Oral Presentation)
저자 및
최봉규, 이남기1,*
포항공과대학교(POSTECH) I-BIO/시스템생명공학부, Korea
1포항공과대학교(POSTECH) 시스템생명공학부/물리학, Korea
Understanding the molecular mechanisms of synaptic vesicle fusion mediated by soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) is essential for studying neuronal communication. Although various in vitro single-vesicle assays monitoring the fusion process were developed before, they have several limitations for detecting the process of SNARE-mediated content mixing and dynamics of fusion pore, transient intermediated steps of vesicle fusion,. Here, we introduce a new, reliable and efficient single-vesicle content mixing assay using a high affinity, fluorophore tagged host-guest pair, cucurbit[7]uril-Cy3 and adamentane-Cy5 as a FRET pair. Using this systems, we can observe the series of conetnt mixing process from vesicle docking, initial fusion pore formation to complete fusion with a high signal to noise ratio. Especially, we successfully observe the reversable flickering of the fusion pore for the first time in vitro. These results demonstrate that the single vesicle content-mixing assay based on our new synthetic host-guest FRET pair is a powerful tool to study the detailed vesicle fusion processes.