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제117회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Structural Investigation into Syndecan Oligomerization Mediated by Its Transmembrane Domain

등록일
2016년 2월 25일 02시 15분 13초
접수번호
1895
발표코드
BIO.P-304 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 21일 (목요일) 11:00~12:30
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
박재현, 이원태*
연세대학교 생화학과, Korea
Syndecans are cell surface adhesion receptors that initiate intracellular signaling events through receptor clustering mediated by their highly conserved transmembrane domain (TMD). Functions of sydecans are controlled by their oligomerization. Syndecans oligomerization is regulated by its TMD. Three constructs of syndecan are designed (Syn-2TMD, Syn-2eTC, Syn-2eTC-F167I mutant) to study on its oligomerization, Syn-2eTC has short additional extracellular and cytoplasmic residues on Syn-2TMD. NMR titration experiments are performed using these constructs, and we observe cross peaks changed after titration. Our results show that the cross peaks of the Syn-2TMD have no significant shift in Syn-2eTC titration experiment. On the other hand, titration of the Syn-2eTC-F167I on Syn-2TMD made dramatic shifts in several cross peaks. It is indicating that Syn-2TMD/Syn-2eTC-F167I titration makes huge conformational changes than Syn-2TMD/Syn-2eTC titration. In detail, F167 and F172 residue of Syn-2TMD showed dramatic chemical shifts in the Syn-2TMD / Syn-2eTC-F167I titration, whereas F169 residue of Syn-2TMD didn’t changed not much. That is larger conformational changes are induced in F167 and F172 versus F169 residue. Also, our structural studies showed that a unique F167 play a important role of interaction between the TMDs of the Syn-2 homo-dimer. UPLC is performed on Syn-2 homo-complex and Syn-2/Syn-2F167I hetero-complex. Syn-2 homo-complex formed higher tetramer formation than Syn-2/Syn-2F167I hetero-complex. Namely, the presence of F167 on TMD was correlated with a higher tendency toward oligomerization. Our work offers new insights into the signaling mediated by the TMD of syndecan family members.

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