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학술발표회초록보기

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  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제117회 대한화학회 학술발표회, 총회 및 기기전시회 안내 A Streamlined Procedure for Selective & Efficient Enrichment of Saliva Glycome

등록일
2016년 2월 18일 15시 05분 31초
접수번호
3108
발표코드
ANAL2.O-12 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 10시 : 20분
발표형식
구두발표
발표분야
분석화학 - 젊은 분석화학자의 구두 발표 II (Oral Presentation of Young Analytical Chemists II)
저자 및
공동저자
윤나영, 김범진, 안현주*
충남대학교 분석과학기술대학원, Korea
Whole saliva is complex biomolecules containing such as a variety of mucin, proteins, DNA, RNA, hormones and electrolytes. Glycoproteins are major of proteins such as α-amylase, mucin, immunoglobulin, lactoperoxidase and lactoferrin in saliva fluid and there are well known as key factor of immune response. A glycosylation change of saliva could reflect the condition of health because glycosylation is highly sensitive to the biochemical environment and it has been implicated in many diseases. However the salivary glycome analysis has not been well studied and were significantly hindered by the inherent component of saliva. Here, we aim to develop the method for more selective and efficient enrichment of glycans directly released from whole saliva. Human saliva were collected from 20 individuals for exploring the salivary glycome. To efficiently eliminate interferences such as electrolytes and small biomolecules, saliva was filtered by 10K MWCO. The supernatants were digested with PNGase F to release N-glycans, then were fractionated according to size and polarity by graphitized carbon solid phase extraction. Enriched glycans were analyzed by MALDI-TOF/TOF MS. We could successfully enrich the N-glycans through pre-filtering procedure in MS analysis, especially, acidic glycan fractions.

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