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  • 03월 02일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제117회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Characterization of Highly Fucosylated N-glycans Found in Human Saliva using LC-MS and LC-MS/MS

등록일
2016년 2월 18일 15시 29분 02초
접수번호
3112
발표코드
ANAL2.O-15 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 10시 : 29분
발표형식
구두발표
발표분야
분석화학 - 젊은 분석화학자의 구두 발표 II (Oral Presentation of Young Analytical Chemists II)
저자 및
공동저자
김범진, 안현주*
충남대학교 분석과학기술대학원, Korea
Saliva, which is secreted mainly from parotid, submandibular, and sublingual glands, contributes to many biological roles and functions in mouth. Saliva is constituted a highly complex mixture such as water, electrolytes, proteins, lipids, and carbohydrates. Glycoproteins are also one of the major components in saliva. Those are involved in the maintenance and protection of oral health against decay, damage, and microbial inflammation. Although studies of profiling of saliva glycoproteins and glycans have been reported, saliva glycosylation has not been well characterized due to inherent complexity and diversity of glycome. In this study, salivary N-glycans have been intensively characterized including not only the compositional and quantitative analysis by nano-LC/MS but also structure elucidation using tandem MS. N-glycans were enzymatically released from saliva after enriching high molecular weight compounds by centrifugation. Solid phase extraction with a porous graphitized carbon cartridge was used to purify and fractionate only N-glycans. We profiled N-glycans using nano LC-chip/Q-TOF MS. Interestingly, we found highly fucosylated glycans (Hex3-7HexNAc3-7Fuc1-7) uniquely present in saliva in contrast with other human fluids such as serum and milk. The structures of novel N-glycans containing 3 to 6 fucoses on outer position were elucidated for the first time by diagnostic fragment ions and fragmentation patterns during tandem MS. Saliva N-glycome may be applied to biomarker discovery of diseases and post-mortem interval estimation in forensic science.

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