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  • 09월 01일 18시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제118회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Inhibitory RNA Aptamers of Tau Oligomerization and Their Neuroprotective Roles against Proteotoxic Stress [우수포스터상]

2016년 8월 31일 18시 09분 19초
BIO.P-153 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 14일 (금요일) 11:00~12:30
저자 및
김지현, 김은경, 이민재*
서울대학교 의과대학 생화학교실, Korea
Alzheimer’s disease is characterized by aggregation of two hallmarks, tau and amyloid beta. Among these misfolded proteins, several studies show that tau aggregation trigger formation of amyloid plaques. Moreover, newly came out researches said that oligomeric tau is more harmful than neurofibrillary tangle (NFT). Until now small molecule tau aggregation inhibitors have been under investigated as potential therapeutic agents against Alzheimer disease. In this study, to find inhibitor of tau oligomerization, we used a systematic evolution of ligands using the exponential enrichment (SELEX) procedure. Here we selected specific anti- tau RNA aptamer 76% homology as named Tau-1. In the binding affinity test, we showed that Tau-1 bounds to tau dose dependently compared with control group. Tau-1 effectively inhibits tau oligomerization, as measured by in vitro tau aggregation assay and thioflavin S assay. We confirmed that Tau-1 doesn’t have any effects on biological events of tau using in vitro transcription/translation. In addition, inhibition of tau oligomerization in cultured cells is also confirmed by using HEK 293 TREX Tau cell and Tau-Bifc cell. To demonstrate inhibitory ability of Tau-1, we identified proteasome and autophagy activity which were not changed. Thus, our study identifies inhibitory Tau-1 aptamer to facilitate inhibition of proteotoxic protein aggregation and may have protential as therapeutic agents for AD pathogenesis.