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  • 09월 01일 18시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제118회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Ultrasensitive Nanoimmunosensor Based on Non-Covalent Functionalized Graphene Oxide Platform and Carbon Nanotubes Bounded Ferritin Labels

등록일
2016년 9월 1일 11시 35분 58초
접수번호
2330
발표코드
ELEC.P-591 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 13일 (목요일) 11:00~12:30
발표형식
포스터
발표분야
전기화학
저자 및
공동저자
Akter Rashida, 정봉진, Md. Aminur Rahman*
충남대학교 분석과학기술대학원, Korea
An ultrasensitive electrochemical nanoimmunosensor for a breast cancer biomarker carbohydrate antigen 15-3 (CA 15-3) was fabricated using non-covalent functionalized graphene oxides (GO/Py-COOH) as sensor platform and multiwalled carbon nanotube (MWCNTs)-bonded numerous ferritin as labels. The immunosensor was constructed by immobilizing a monoclonal anti-CA 15-3 antibody on the GO modified cysteamine (Cys) self-assembled monolyer (SAM) on an Au electrode (Au/Cys) through the amide bond formation between the carboxylic acid groups of GO/Py-COOH and amine groups of anti-CA 15-3. Secondary antibody and ferritin conjugated MWCNT bioconjugates (Ab2-MWCNT-Ferritin) were prepared through the amide bond formation between amine groups of Ab2 and ferritin and carboxylic acid groups of MWCNTs. The detection of CA 15-3 was achieved through monitoring the ferritin catalyzed hydrogen peroxide reduction at the GO/Py-COOH-based sensor probe. The GO/Py-COOH-based sensor probe and Ab2-MWCNT-Ferritin bioconjugates were characterized using various techniques. Using differential pulse voltammetry (DPV), CA 15-3 can be selectively detected as low as 0.01 ± 0.07 U/mL in human serum samples with excellent stability, which demonstrated that the proposed nanoimmunosensor has potentials in proteomic researches and diagnostics.

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