KCS

The electromembrane extraction(EME) is one of the liquid phase microextraction(LPME) which can extract and concentrate the analyte from aqueous matrices such as urine and plasma effectively. This method was applied to analysis of six Non-steroidal anti-inflammatory drugs(NSAIDs) in aqueous samples. The parameters such as pH of acceptor and sample, stirring speed, voltage, support liquid membrane(SLM), and extraction time were optimized. Simultaneous chromatographic separation of six NSAIDs by HPLC-UV/Vis system was achieved on an Eclipse XDB-C18 (4.6 mm i.d. × 150 mm length, 5 ㎛ particle size) column using gradient elution with 0.1 % formic acid and methanol. 0.1 % formic acid and methanol start to 20:80 and after 7minutes, compositon and flow rate are changed to 15:85, finally, back to the first condition after 5minutes. After the extraction, 5 ㎕ of extract was directly injected into the HPLC-UV system.