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학술발표회초록보기

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제118회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Relative Quantitation of N-linked Glycans in Rice using Metabolic Isotope Labeling by Mass Spectrometry

등록일
2016년 8월 25일 14시 54분 47초
접수번호
3112
발표코드
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발표시간
금 09시 : 27분
발표형식
구두발표
발표분야
분석화학 - Oral Presentation of Young Analytical Chemists Ⅱ
저자 및
공동저자
서현경, 김진화, 임재민*
창원대학교 화학과, Korea
Glycosylation is one of the most common post-translational modifications in biological system. Glycan play significant roles for a wide range of biological processes regulating such as cell-cell interactions, cell division and adhesion, cellular recognition, immune response, and protein stability. For the purpose of understanding the biological relevance of glycans, many mass spectrometry based methods have been developed for the relative quantitation of glycan. Herein, we describe a new in vivo labeling method either normal or heavy glucose to tag glycans from rice culture. Isotopic glucose enters the glucose metabolic pathway, which in turn produces nucleotide sugars precursors that are subsequently used for all glycan biosynthesis. Thus, each of sugars in glycan occur a mass shift as much as 1 Da. For glycan containing five sugars, occurs a mass shift of as much as 5 Da. A method for relative quantitation of glycans by ratio of normal glucose labeled glycan and heavy glucose labeled glycan.

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