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  • 09월 01일 18시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제118회 대한화학회 학술발표회, 총회 및 기기전시회 안내 SERS-based Wash-free Magnetic Immunoassay Using Sequential Droplet Merging and Splitting

2016년 8월 25일 15시 42분 23초
ANAL2.O-17 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 09시 : 48분
분석화학 - Oral Presentation of Young Analytical Chemists Ⅱ
저자 및
최남현, 고주희1, 주재범2,*
한양대학교 바이오나노공학과, Korea
1한양대학교 나노센서연구소, Korea
2한양대학교 생명나노공학과, Korea
The combination of surface-enhanced Raman scattering (SERS) with microfluidic devices provides an ideal mechanism for achieving rapid, sensitive and reproducible detection of specific biomarkers in solution. An additional benefit of such platform is the use of minimal sample volumes and low analyte concentrations. We recently reported a SERS-based wash-free magnetic immunoassay technique using magnet-embedded droplet microfluidics. In this platform, the assay could be performed without any washing process by separating immunocomplexes from the supernatant solution using a magnetic separation. Nonetheless, this methodology still limited in assay because the channel structure for reagents injection and mixing is unified in a channel. To resolve this problem, a new class of microdroplet-merging device was designed and fabricated for sequential immunoassay. This device is composed of five distinct compartments such as droplets merging, separating immunocomplexes from supernatant solution, isolating magnetic immunocomplex droplets, and collecting unbound SERS probe droplets for detection. Herein, we report a novel SERS-based wash-free immunoassay device composed of compartments for mixing, merging, splitting and detection.