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학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

현재 가능한 작업은 아래와 같습니다.
  • 09월 01일 18시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제118회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Novel Strategy for Quality Assessment of Glycosylation on a Biotherapeutic Glycoprotein by Intact Protein Analysis

등록일
2016년 8월 25일 15시 44분 19초
접수번호
3121
발표코드
ANAL2.O-18 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 09시 : 51분
발표형식
구두발표
발표분야
분석화학 - Oral Presentation of Young Analytical Chemists Ⅱ
저자 및
공동저자
김운용, 오명진1, 서영숙2, 문한태2, 안현주2,*
충남대학교 AGRS, Korea
1충남대학교 분석과학대학원, Korea
2충남대학교 분석과학기술대학원, Korea
Protein glycosylation plays an important role in determining in vivo bioactivity, stability, solubility, and metabolic fate of biotherapeutics. Thus, detailed glycomic analyses are necessary complement to ensure quality, safety, and potency of therapeutic glycoproteins during production process. Current methods such as bottom-up and middle-down approaches employing LC-MS and LC-MS/MS for the glycomic characterization of biotherapeutics provide valuable information regarding glycan structure, isomers, and micro-heterogeneity. However, these methods significantly demand massive time and efforts from sample preparation to data interpretation. For these reasons, glycan or glycopeptide based approach is not suitable for real-time quality control in manufacturing processes. This study aims to develop novel analytical tool for real time determination of glycosylation site-occupancy with micro-heterogeneity using intact protein analysis (native mass spectrometry) without any additional sample preparation steps which can provide comprehensive glycosylation information.

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