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  • 09월 01일 18시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제118회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Salivary Glycomic Approach for the Distinction of Human Constitutions

2016년 8월 25일 15시 47분 31초
ANAL2.O-20 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 09시 : 57분
분석화학 - Oral Presentation of Young Analytical Chemists Ⅱ
저자 및
문한태, 윤나영, 김범진, 안현주*
충남대학교 분석과학기술대학원, Korea
Unlike western medicine, oriental medicine has the constitutional diagnostics to determine individual health status. The accurate diagnosis of constitution is very important because therapy can be differed from patient’s constitution. Saliva, readily accessible and noninvasive fluid, contains mucins, which are heavily glycosylated proteins. Changes of glycosylation reflect health status because glycosylation is highly sensitive to the biochemical environment. In this study, we aim to conduct MS-based salivary glycomic approach to identify potential glycan signature for constitution classification. Saliva was collected from 9 normal people; 7 pancreotonia, 1 pulmotonia and 1 colonotonia. Initially, the saliva was filtered by 10K MWCO spin column to remove impurities. N-glycans were enzymatically released by PNGase F and purified by C18 SPE. Finally, the N-glycans were eluted by PGC SPE. For overall N-glycan profiling, both MALDI-TOF MS and nano-LC/Q-TOF MS were used to provide independent orthogonal analysis. Approximately 150 compositions of N-glycan were profiled and they were compared to identify the difference of glycans depending on presence (frequency), quantitation, composition, and structure. In constitution distinction, bi- and tri-antennary fucosylated N-glycans are high in abundance in pulmotonia and colonotonia while sialylated N-glycans are high in abundance in pancreotonia. MS-based salivary glycomic approach can be used as a discrimination system for the human constitution classification.