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제118회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Simultaneous Detection of Dual Prostate Cancer Markers in Blood Serum Using SERS-Based Immunoassay

등록일
2016년 8월 25일 15시 57분 30초
접수번호
3125
발표코드
ANAL2.O-22 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 10시 : 03분
발표형식
구두발표
발표분야
분석화학 - Oral Presentation of Young Analytical Chemists Ⅱ
저자 및
공동저자
CHENGZIYI, 주재범1,*
한양대학교 바이오나노공학과, China
1한양대학교 생명나노공학과, Korea
Prostate-specific antigen (PSA) is a 30-kDa serine protease. PSA in blood exists in dual forms; one is a free form and the other is a complexed form with the protease inhibitors a1-antichymotrypsin (ACT). Total PSA is a combination of both free and PSA/ACT complexed forms. Previous reports show that the clinical accuracy of PSA biomarkers can be improved by measuring the ratio between free-PSA and PSA-ACT in serum. In this work, we developed a novel SERS-based immunoassay technique for the simultaneous detection of both PSA/ACT and free-PSA biomarkers in serum. For this purpose, total-PSA antibody-conjugated magnetic beads and two different types of antibody-conjugated SERS nano-tags (MGITC labeled GNP conjugated with free-PSA monoclonal antibody (mAb) and XRITC labeled GNP conjugated with PSA/ACT mAb) were prepared. The assay was performed by the following procedure. First, total PSA antibodies were immobilized on the surface of a carboxyl-modified magnetic bead. Second, different ratios of PSA/ACT and free-PSA mixtures were prepared and added to the antibody-conjugated magnetic bead solution. Third, two different types of SERS nano-tags were added to the solution. Finally, the duplex sandwich immunocomplexes were isolated by a magnetic bar, and then the SERS signals of SERS nano-tags were measured and analyzed. This assay platform will be useful for the early diagnosis of prostate cancer patients.

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