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제118회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Study on stability of lipid vesicle using asymmetrical flow field-flow fractionation (AsFlFFF) and dynamic light scattering (DLS)

2016년 8월 25일 16시 44분 13초
ANAL2.O-26 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 10시 : 15분
분석화학 - Oral Presentation of Young Analytical Chemists Ⅱ
저자 및
김민욱, 최재영, 한수정, Lars Nilsson1, 이승호*
한남대학교 화학과, Korea
1Lund University, Sweden
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승인 1건
Vesicles are of microscopic structure and composition that enclose a volume with a molecularly thin membrane. Generally the membrane is a self-directed assembly of amphiphilic molecules with a dual hydrophilic-hydrophobic nature, and is a spherical shell composed of lipid bilayer that encapsulate an aqueous space. Vesicles have been used as nano-scale containers for drugs, model membranes and other substances in pharmaceutical and cosmetic industries. Generally the stability of vesicles is closely related with their size and size distribution. When vesicles are used for drug delivery, the stability, and thus their life-time is directly associated with the medicinal effects. Thus size characterization of vesicles is necessary for understanding of the vesicle stability. Various techniques including electronic microscopy (EM), nuclear magnetic resonance (NMR), dynamic light scattering (DLS) and nanoparticle tracking analysis (NTA) have been used for size analysis of nano-sized particulates. DLS is simple to use, and has been widely used for analysis of particle size and its distribution. The application of DLS is however rather limited when dealing with samples of complex mixtures or of broad size distributions. Field-flow fractionation (FFF) has been employed for size-based separation and size-characterization of particles having broad size distributions from a few nm up to about 100 μm. Asymmetrical flow field-flow fractionation (AsFlFFF), one of members of FFF family, is particularly useful for characterization of aqueous colloidal particles. In this study, nano-sized vesicles were prepared by extrusion with the lipid concentration varied from 0.5 to 1.5%, and then were analyzed by AsFlFFF for determination of the size distribution, the stability (and life-time) and the osmotic pressure. It was found that the stability of vesicle increases as the lipid concentration increases. It was also observed that the vesicle stability is affected by the osmotic pressure.