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학술발표회초록보기

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  • 02월 19일 23시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

대한화학회 제119회 학술발표회 및 총회 Flow FFF with Mass Spectrometry for Proteomic and Lipidomic Analysis

등록일
2017년 1월 31일 11시 04분 52초
접수번호
2601
발표코드
ANAL1-1 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 13시 : 30분
발표형식
심포지엄
발표분야
분석화학 - Recent Advances in Analytical Chemistry I: Advanced Materials and Instrument in Analytical Chemistry
저자 및
공동저자
문명희
연세대학교 화학과, Korea
Flow field-flow fractionation (FlFFF) is a separation method capable of size-sorting particles and biological macromolecules like proteins, DNA, cells, and etc. in an empty channel space without packing materials. During separation of macromolecules by FlFFF, eluting fractions of narrow size range can be collected and utilized for further biological analysis. In this presentation, recent efforts to incorporate mass spectrometry (MS) with FlFFF either by off-line or on-line will be described with applications to proteomic and lipidomic analysis. For the case of off-line combination of FlFFF and MS, subcellular and extracellular species were separated by sizes first and then the collected organelle fractions of different sizes can be further investigated for the proteomic and lipidomic analysis using nanoflow LC-ESI-MS/MS. A miniaturiaed FlFFF can be utilized for the direct hyphenation to MS via electrospray ionization (ESI) or inductively couple plasma (ICP). The former enabled one to have top-down analysis of lipids from lipoproteins without extracting lipids and the latter led us to analyze metals from metalloproteins in blood plasmsa proteome. This presentation introduces recent works on the proteomic/lipidomic analysis of urinary exosomes from prostate cancer patients and on the direct metal analysis of metalloproteins from plasmsa samples with lung cancer.

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