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  • 02월 19일 23시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

대한화학회 제119회 학술발표회 및 총회 Heavy Water Labeling : Extracting a Big Change from Small Variations

등록일
2017년 2월 8일 18시 00분 30초
접수번호
3055
발표코드
ANAL1-6 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 15시 : 20분
발표형식
분과기념
발표분야
분석화학 - Recent Advances in Analytical Chemistry I: Advanced Materials and Instrument in Analytical Chemistry
저자 및
공동저자
김태영
GIST 지구환경공학부, Korea
Mass spectrometry (MS) has revolutionized biochemical analysis in terms of sensitivity, specificity, and speed. A fundamental drawback of MS as an analytical technique is the poor ability of quantification. To overcome this limitation, stable isotope labeling has been extensively employed for both relative and absolute quantification based on MS. In most quantitative MS experiments, complete labeling of target analytes with a specific type of heavy isotope (e.g., 2H, 13C, or 15N) is designed for simple distinction of isotope-labeled compounds from non-labeled counterparts in a mass spectrum. However, the use of isotope labeling is a cumbersome practice mainly due to high cost and long time to achieve complete labeling. An alternative method to introduce an isotope to biomolecules is indirect deuterium labeling via heavy water (2H2O) administration, which results in strikingly different patterns of mass spectra because of partial isotope enrichment. In this presentation, properties and characteristics of 2H2O labeling are discussed along with its limitations and challenges for applications in MS-based quantification studies.

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