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  • 02월 19일 23시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제119회 대한화학회 학술발표회, 총회 및 기기전시회 Characterization and mutational analysis of a cold-adapted bacterial lipase, SaHSL from Salinisphaera sp. P7-4 [TCI 포스터상]

등록일
2017년 2월 10일 14시 33분 16초
접수번호
3891
발표코드
BIO.P-307 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
4월 21일 (금요일) 13:00~14:30
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
유완기, 김부영1, 김두헌2,*
성균관대학교 의과대학, Korea
1숙명여자대학교 화학과, Korea
2숙명여자대학교 이과대학 화학과, Korea
Hormone sensitive lipase (HSL) contributes energy metabolism and fatty acid signaling in various tissues. Functional and structural properties of HSL from human have been studied in detail, however, lipase homologues from bacteria are still largely unknown. Previously, a new cold-adapted bacterial lipase, SaHSL was identified and purified. Here, biochemical and biophysical studies of SaHSL were reported. Primary structure analysis of SaHSL revealed that SaHSL was different from human HSL, especially in N terminal region and regulatory domain. Here, SaHSL were investigated with fluorescence analysis, dynamic light scattering (DLS), electron microscopy, and time of flight (TOF) mass spectrometry. Studies of site-directed mutants in regulatory region suggested that the region are critical for structural stability as well as for substrate specificity. Improvement of chemical, thermal resistance and good durability of immobilized SaHSL proposed the potential as a biocatalyst in industries.

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