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  • 02월 19일 23시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제119회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Liquid extraction surface analysis coupled with commercial capillary electrophoresis for direct analysis of solid surface samples rapidly

2017년 2월 16일 14시 23분 32초
ANAL.P-264 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
4월 21일 (금요일) 13:00~14:30
저자 및
정선경, 정두수1,*
서울대학교 화학부, Korea
1서울대학교 화학부, Korea

Capillary electrophoresis (CE) is well suited for the analysis of analytes in aqueous solutions. It is not easy to analyze a solid sample with CE since labor-intensive sample pretreatment processes are required. For example, a solid sample should be grounded, homogenized, centrifuged, extracted into an organic solvent, and then reconstituted into an aqueous solution suitable for CE. So we used liquid extraction surface analysis (LESA) that extracts chemicals on the surface of various solid samples without any sample pretreatment. Dried analytes doped on a surface were directly extracted to a hanging drop of extractant at the inlet tip of a capillary. After extraction, a small volume of acceptor was injected into the capillary and analyzed by a commercial CE instrument. LESA-CE was used to determine three species of insecticides, chlorantraniliprole, kresoxim-methyl, and pyraclostrobin. The U.S. Environmental Protection Agency (EPA) limits the allowable amounts of the insecticides to 4.0 ppm, 0.5 ppm, and 2.5 ppm respectively. For these water-insoluble analytes, micellar capillary electrophoresis (MEKC) was used and an on-line stacking method, analyte focusing by micelle collapse (AFMC) was combined to enhance the sensitivity. These three pesticides doped on sample plate were extracted with the inlet of a capillary and analyze directly with CE. Several parameters such as the condition of AFMC-MEKC, composition of acceptor, extraction time were optimized. The limits of detection (LODs) with LESA-AFMC-MEKC were 72, 23, and 29 ppb, respectively. Thus LESA-CE is a simple, sensitive and more rapid method for the analytes solid surface analytes without any pretreatment processes required for conventional analysis methods. The developed LESA-CE of high spatial resolution and immediate quantification ability is promising for the analysis of biological surfaces of cells and tissue, or even technical surfaces.