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학술발표회초록보기

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제120회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Dark-Field Illumination-Based Enhanced Fluorescence-free 3D Super-Resolution Microscopy

등록일
2017년 8월 27일 10시 06분 45초
접수번호
2160
발표코드
ACBI-9 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 15시 : 00분
발표형식
심포지엄
발표분야
Analytical Chemistry-Life Chemistry - [Analytical Chemistry Division - JASIS (Japan) - Life Chemistry Division Joint Symposium] Super-Resolution Optical Microscopy and Single-Cell Analysis
저자 및
공동저자
Seong Ho Kang
Department of Applied Chemistry, Kyung Hee University, Korea
Fluorescence-free three-dimensional (3D) super-resolution microscopy (SRM) was developed based on wavelength-dependent dark-field illumination and a least-cubic algorithm. Various plasmonic nanoparticles on a glass slide (i.e., gold nanoparticles, GNPs; silver nanoparticles, SNPs; and gold nanorods, GNRs) were imaged and sliced in the z-direction to a thickness of 10 nm. The 3D coordinates of individual GNP, SNP, and GNR (x, y, z) were resolved by fitting the data with 3D point spread functions using a least-cubic algorithm and collation. Final, 3D SRM images were obtained by resolving 3D coordinates and their Cramér-Rao lower bound-based localization precisions in an image space (530 nm × 530 nm × 300 nm) with a specific voxel size (2.5 nm × 2.5 nm × 5 nm). The least-cubic method was more useful for finding the center in asymmetric cases (i.e., nanorods) with high precision and accuracy compared with the commonly used least-square method. This fluorescence-free 3D SRM technique was also successfully applied to resolve the positions of various nanoparticles in live single cells and nanobiochips with subdiffraction limited resolution in 3D.

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