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제120회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Electrochemical immunosensors using fragmented antibody (Fab’) and electrochemically active nanoparticles for diagnosis of allergic rhinitis

등록일
2017년 8월 31일 16시 42분 25초
접수번호
2450
발표코드
ELEC.P-499 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 20일 (금요일) 13:00~14:30
발표형식
포스터
발표분야
Electrochemistry
저자 및
공동저자
Sanggyeong Shin, Kihak Gwon, Jae Ho Shin*
Department of Chemistry, Kwangwoon University, Korea
Electrochemical immunosensors based on antibody provide a rapid and convenient approach for sensitive and specific disgnosis. Despite many advantages, it is still a challenge to find new approaches that could improve the simplicity, specificity, and sensitivity for clinical uses. To enhance performance of antibody based immunosensors, it is particularly important to develop label-free detection methods. Several strategies to achieve label-free immunoassays have been demonstrated by using fluorescence, electrochemistry, quartz crystal microbalance (QCM), and surface plasmon resonance (SPR) spectroscopy. Herein we reported on a novel approach to design a label-free immunosensors via use of electrochemically active nanoprobes such as ferrocene (Fc)-modified silica nanoparticles. The fragmented antibody (i.e., Fab’; regular molecular weight of 50 kDa) immobilized on a gold electrode leads to changes in the interfacial electron transfer properties. The surface coverage with target proteins specifically bound to Fab’ prohibits the access of electroactive probes to the electrode surface. To amplify such blocking effects associated with target protein-Fab’ complex formation, Fc-modified silica nanoparticles are utilized as a scaffold to design macromolecular electroactive probes. Various conjugation techniques and addressed fabrication methods for Fab’ oriented immobilization on a gold surface will be evaluated. Furthermore, we investigate the feasibility of applying the novel concept to develop the label-free, Fab’-based immunosensor to detect the biomarkers in allergic rhinitis such as Clara cell protein 16, tryptase, eosinophil cationic protein, and albumin. Finally, four biomarkers in artificial nasal discharge are simultaneously determined with multi-array sensor system equipped in a microfluidic device.

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