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  • 09월 05일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제120회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Optimization of Electrical Communication for Direct Electron Transfer of Glucose Dehydrogenase Immobilized Electrode

등록일
2017년 8월 31일 16시 51분 44초
접수번호
2452
발표코드
ENVR.P-525 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 19일 (목요일) 11:00~12:30
발표형식
포스터
발표분야
Environmental Energy
저자 및
공동저자
Hyeryeong Lee*, Yoo Seok Lee
Division of Environmental Engineering, Gwangju Institute of Science and Technology, Korea
Enzymatic biofuel cells are promising technology which can consume complex fuels (glucose, sucrose, etc) which is abundantly distributed in the nature, and can operate in mild pH and temperature range. The efficient electron transfer (ET) between the redox active site of the protein and the electrode surface is the main challenge for the development of high performance of enzymatic fuel cells. For the ideal ET conditions, direct ET (DET) system is needed to be constructed to exclude thermodynamic losses. So, binding stability of enzyme to electrode surface, distance of active site-electrode surface, and charge transfer resistance are the focusing issues as parameters related to DET. To optimize binding stability of enzyme to electrode and ET distance of enzyme-electrode, gold binding peptide (GBP) was site-specifically expressed via the genetic engineering of the α-subunit of glucose dehydrogenase (GDH), enabling DET between enzyme and electrode surface, as well as stable immobilization on electrode. In addition, lateral steric hindrance of neighboring enzyme is another considering point for optimization of electrical communication between enzyme and electrode surface because enzymatic steric hindrance can decrease contact area of active site on electrode and increases resistance on charge transfer processes. In this study, the influence of enzymatic steric effects on DET efficiency was investigated and proved by utilizing nano-fabricated matrix. Based on the result, it was proposed that lateral steric hinderance among enzymes should be considered as important parameter for DET and nano-fabricated electrode is necessary to control enzymatic steric effect for development of efficient enzymatic fuel cell.

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