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  • 09월 05일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제120회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Phase Determination of Iron-Dependent Homogentisate Dioxygenase from Acinetobacter oleivorans

등록일
2017년 8월 31일 17시 12분 41초
접수번호
2466
발표코드
BIO.P-290 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 19일 (목요일) 11:00~12:30
발표형식
포스터
발표분야
Life Chemistry
저자 및
공동저자
Suk-Youl Park*, Seung-A Hwangbo
Structural Biology Group, Pohang Accelerator Laboratory, Korea
In aromatic compounds mineralizing bacterium Acinetobacter oleivorans, homogentisate 1,2-dioxygenase (aoHGO) catalyzes the conversion of homogentisate to 4-maleylacetoacetate by aromatic ring scission using zinc ions, in the breakdown of tyrosine and phenylalanine. To determine the molecular background of the enzymatic mechanism of HGO in this iron-resistant organism, DH17_10945 encoding aoHGO of Acinetobacter oleivorans was cloned, and the expressed protein was purified. The protein was crystallized in 0.2 M sodium chloride, 25% [w/v] polyethylene glycol 3350 and 0.1 M BIS-TRIS at pH 6.5. X-ray diffraction data were collected to 1.5 Å resolution using synchrotron radiation. The crystal belongs to the orthorhombic space group C2221, with unit cell dimensions of a = 53.1 Å, b = 121.2 Å, and c = 54.4 Å. A traceable electron density map was calculated using anomalous diffraction data obtained from a crystal soaked in zinc ions.

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