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  • 09월 05일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제120회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Chromatographic Enantiomer Separation of Chiral Amines as Nitrobenzoxadiazole Derivatives on Several Polysaccharide-Derived Chiral Stationary Phases by Normal HPLC under Simultaneous Ultraviolet and Fluorescence Detection

2017년 8월 19일 21시 49분 25초
ANAL1.O-16 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 09시 : 46분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists I
저자 및
Adhikari Suraj, Wonjae Lee*
College of Pharmacy, Chosun University, Korea
A convenient and derivatized method using a fluorogenic agent, 4-chloro-7-nitro-1,2,3-benzoxadiazole (NBD-Cl) was developed for enantiomer resolution of chiral aliphatic amines including amino alcohols by normal HPLC. The enantiomer separation of chiral amines as NBD derivatives was performed on six covalently bonded and four coated type polysaccharide-derived chiral stationary phases (CSPs) under simultaneous ultraviolet (UV) and fluorescence detection (FLD). Among the covalently bonded CSPs, Chiralpak IE showed the best enantiomer separation for most analytes. The other CSPs also showed good enantioselectivity except for Chiralpak IB. On the other hand, Chiralpak AD-H and Amylose-1 generally exhibited better enantiomer separation of NBD derivatized chiral amines among the coated CSPS. The developed analytical technique was also applied to determine the optical purity of commercially available (R)- and (S)-leucinol; the impurity was found to be 0.06%. The developed method was validated and proved to be an accurate, precise, sensitive and selective method suitable for enantiomer separation of chiral aliphatic amines as NBD derivatives under simultaneous UV and fluorescence detection.