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09월 05일 17시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능
제120회 대한화학회 학술발표회, 총회 및 기기전시회 안내
Forensic Platform for Identification of Human Saliva using MS-based Glycomics
2017년 8월 21일 17시 17분 24초
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목 09시 : 48분
Analytical Chemistry -
Oral Presentation of Young Analytical Chemists I
, Bum Jin Kim
, Hyun Joo An
Graduate School of Analytical Science and Technology, Chungnam National University, Korea
In forensics, saliva found at crime scenes is one of the important evidences and thus, the identification of human saliva from other human fluids and non-human fluids, respectively is an essential prerequisite prior to further crime investigation. In previous study, we could determine that the degree and amount of highly fucosylated N-glycans were specific features to identify human saliva from other body fluids through MS-based glycomic approach. However, most saliva encountered at crime scenes has a trace amount and has been often found as a dry spot. For these reasons, forensic platform with high sensitivity for identification of human saliva is required. Herein, we have developed MS-based platform using glycomics and characterized N-glycans extracted from a trace amount of dried saliva. Briefly, dried saliva was prepared by spotting the saliva onto a protein saver card and drying at ambient temperature. N-glycans were enzymatically released by PNGase F from dried saliva spot (DSS) and enriched by solid phase extraction with a porous graphitized carbon cartridge. Then, saliva N-glycans were characterized by MALDI-TOF/TOF MS. N-glycans found in DSS showed a high correlation in terms of the number of glycans and their amount compared with N-glycans released from saliva fluid. Indeed, highly fucosylated N-glycans were also observed as a specific signature in DSS. We also examined technical validated reproducibility of N-glycan analysis based on DSS. This is the first study to apply a glycomic tool to forensic science.
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