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제120회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Steric Transition Phenomena upon Field Decay Patterns Using Frit-inlet Asymmetrical Flow Field-Flow Fractionation

등록일
2017년 8월 22일 15시 56분 30초
접수번호
3023
발표코드
ANAL1.O-26 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 10시 : 11분
발표형식
구두발표
발표분야
Analytical Chemistry - Oral Presentation of Young Analytical Chemists I
저자 및
공동저자
Young Beom Kim*, Lee Hye Jin, Myeong Hee Moon*
Department of Chemistry, Yonsei University, Korea
Flow field-flow fractionation (FlFFF) is a separation technique that can separate macromolecules by size without stationary phase. The separation mode of FlFFF can be divided into normal and steric/hyperlayer mode; particles with sizes smaller than 1µm are operated in normal mode while those larger 1µm are operated in steric/hyperlayer mode. During the focusing/relaxation process, smaller particles diffuse more and equilibrate at a position higher from the channel than larger particles. As a result, The smaller particles are located in faster flow stream and eluted earlier than the larger particles. In the steric/hyperlayer mode, where the particle size is larger than 1µm, Brownian diffusion becomes negligible and particles are elevated to a certain height by lift force. Since the larger particles have higher lift forces than the smaller particles, they elute earlier. Steric transition is a phenomenon that particle elutes by a combination of normal mode and steric mode in FlFFF. Frit-inlet asymmetrical flow field-flow fractionation (FI-AF4) operates by stopless flow without focusing process. In this study, steric transition phenomenon is systematically monitored with polystyrene particles by FI-AF4 coupled with UV/visible detector and multi-angle light scattering. Depending on the strength and gradient of the field, the particle size at which the steric transition occurs varies. Therefore, it is possible to increase the range of particle size separated in the normal mode.

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