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제120회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Highly sensitive detection of lethal infectious pathogen using SERS-based lateral flow assay

등록일
2017년 8월 31일 09시 52분 51초
접수번호
3129
발표코드
ANAL2.O-32 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 10시 : 23분
발표형식
구두발표
발표분야
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
공동저자
Rui Wang, Kihyun Kim, JAEBUM CHOO*
Department of Bionano Technology, Hanyang University, Korea
Effective identification of pathogenic bacteria is important for biodefense. In recent years, microbiological culture and colony counting, polymerase chain reaction (PCR) or immunoassay has been considered as a gold standard for the detection of pathogens including toxin, bacteria and virus. However, these methods cannot meet the requirement of rapid and highly sensitive detection because they need long sample preparation time and have limited detection sensitivity. Therefore, it is still urgent to develop a new method for the rapid and sensitive detection of pathogens for preparedness of bioterrorism. In this presentation, we introduce a new pathogen assay technique using a surface-enhanced Raman scattering (SERS)-based lateral flow assay. Two different antigens, including botulinum toxin A and Yersinia pestis have been selected as detection targets. According to our assay results, the limit of detection of botulinum toxin A and Yersinia pestis using the SERS-based lateral flow platform was 10 pg/mL and 102 CFU/mL, respectively, which are three orders of magnitude more sensitive than that of commercial rapid kit. Thus, the proposed SERS-based lateral flow assay has a strong potential to be a valuable tool in the early detection of a specific pathogen due to its excellent analytical sensitivity.

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