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제120회 대한화학회 학술발표회, 총회 및 기기전시회 안내 A Method for Quantitative Analysis of Cellular Uptake in Combination Therapy Treating Neuroblastoma

2017년 8월 31일 11시 19분 05초
ANAL2.O-37 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 10시 : 33분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
Hong Areum, Min Gyeongseo, Hugh Inkon Kim*
Department of Chemistry, Korea University, Korea
Neuroblastoma is fourth most abundant malignant tumor in childhood which begins when immature nerve cells grow out of control. As Neuroblastoma belongs to heterogeneous disease, chemotherapy has been underway as combination of various anticancer drugs. Therefore, to optimize combination chemotherapy for diverse case, understanding how combination therapy works better than single drug is crucial. However, relevant researches so far have been focused on only combination efficiency in clinical investigation. In this research, we aim to study cellular uptake of combination therapy in correlation to drug efficiency for cancer cells, compared to single drug regimen. Firstly, cell viability test for neuroblastoma cell lines, SK-N-SH and SH-SY5Y, was conducted using each drug in combination therapy to search proper drug treating condition. Also, we tried to develop quantitation method based on mass spectrometry for drug in cell culture media. As a result, we optimized solvent condition for maximizing drug ionization efficiency in mass spectrometry and pretreatment method of culture media to quantify small molecules such as drugs. With aforementioned results, we are currently working on the correlation between treatment efficiency and time-based cellular uptake after using combination or single drug.