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  • 02월 19일 10시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제121회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Heterometal coordinated monomeric concanavalin A at pH 7.5

2018년 2월 1일 15시 52분 05초
INOR.P-35 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
4월 19일 (목요일) 11:00~12:30
Inorganic Chemistry
저자 및
Xingchen Han, Seung Jae Lee*
Department of Chemistry and Research Institute for Molecular Biology and Genetics, Chonbuk National University, Korea
The structure of concanavalin A (ConA) has been studied intensively due to its specific interactions with carbohydrates and its heterometal (Ca2+ and Mn2+) coordination. Biophysical studies on ConA and pathogen glycoproteins have provided details on their interactions for the field of diagnostic microbiology, and these studies can be extended for detecting and estimating the concentration of viruses. Studies on the structure of ConA from Canavalia ensiformis, the jack bean, have demonstrated crucial details, including interaction residues such as hydrogen bonds and salt-bridges with carbohydrates. Reports have shown that heterometal ions, including manganese and calcium ions, are crucial for this interaction with carbohydrates. Most structures from X-ray crystallography have shown ConA as a dimer or tetramer, because the complex formation requires specific crystallization conditions. Here, we reported a monomeric structure of ConA with a resolution of 1.6 Å, which revealed that metal coordination could trigger sugar binding ability. The calcium coordination residue, Asn14, changes the orientation of carbohydrate binding residues and biophysical details, including structural information, providing valuable clues for the development and application of detection kits using ConA.