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  • 02월 19일 10시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제121회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Interaction mechanisms of protein-human norovirus induced by divalent metal coordination

2018년 2월 2일 11시 01분 48초
INOR.P-41 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
4월 19일 (목요일) 11:00~12:30
Inorganic Chemistry
저자 및
Jaewoong Park, Seung Jae Lee*
Department of Chemistry and Research Institute for Molecular Biology and Genetics, Chonbuk National University, Korea
Noroviruses (NoV) are non-enveloped single-stranded positive-sense RNA viruses belonging to the Caliciviridae family, and are divided into seven genogroups (GI–GVII) based on viral capsid gene sequences. The genotypes in the genogroups GI, GII, and GIV, referred collectively as human norovirus (HuNoV), are major causes of acute water- and food-borne global outbreaks of viral gastroenteritis. HuNoV is responsible for 90% of viral gastroenteritis, and 50% of all gastroenteritis outbreaks; this virus causes acute diarrhea and vomiting, which typically resolve within 2 or 3 days, but can result in life-threatening dehydration in children and the elderly. Rapid methods for the detection and clinical treatment of human norovirus (HuNoV) are required for controlling foodborne disease outbreaks, but a reliable methodology that is fast and sensitive enough to detect small amounts of HuNoV in food and aquatic environments has not been available. We report the interaction details of HuNoV with concanavalin A (ConA) which aid in the development of a sensitive detection tool for HuNov. Biophysical studies revealed that when the metal coordinated region (MCR) of ConA, which spans Asp16 to His24, is converted to nine alanine residues (mConAMCR), the affinity of the interaction with HuNoV (GII.4) diminishes, demonstrating that this Ca2+ and Mn2+ coordinated region is responsible for this virus-protein interaction. Furthermore, ConA conjugated polyacrylate beads (ConA-column) showed good recovery rates of GI and GII types of HuNoV from food items over a broad range of pH (3.0 ~ 10.0). Utilization of ConA allows for the development of a rapid and sensitive detection and concentration methodology for diverse genotypes of HuNoV.