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  • 02월 19일 10시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

대한화학회 제121회 학술발표회 및 총회 Multicolor Quantum Dot Labeling of Both of Hydrophilic and Hydrophobic Regions of Liposome

2018년 2월 7일 10시 26분 17초
INOR.P-110 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
4월 19일 (목요일) 11:00~12:30
Inorganic Chemistry
저자 및
Yeonki Hong, Daeha Seo*
Department of Emerging Materials Science, Daegu Gyeongbuk Institute of Science & Technology, Korea
It is now recognized that plasma membrane regulating all protein functions by generating a variety of micro- domains. Despite importance of plasma membrane in spatiotemporal dynamics, direct imaging probe to observe the lipid bilayer in live cell is limited compared with other membrane receptors and their compartments, because the imaging probe should overcome the chemical potential between hydrophilic and hydrophobic layers. Here, we constructed probe delivery systems to the plasma membrane by generating hybrid nanostructures composed of liposome and inorganic nanocrystals, quantum dots (QDs). Since the thickness of lipid bilayer is about 4 nm, QDs are suitable optical probe which is typically between 2 and 10 nm in size. We prepared small unilamellar vesicles (SUVs) composed of trioctylphosphine oxide-coated green QDs embedded in lipid bilayer and mercaptopropionic acid-coated red QDs encapsulated in SUV. Fluorescence microscopy images of the SUVs show a colocalization of hydrophobic and hydrophilic QDs. Such system should find broad utility in studies requiring single-molecule imaging either in cytosolic or in lipid membrane.