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02월 19일 10시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능
제121회 대한화학회 학술발표회, 총회 및 기기전시회 안내
Optimization of method for marker compound analysis of Aster glehni extracts using HPLC
2018년 2월 13일 11시 58분 58초
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4월 19일 (목요일) 11:00~12:30
, Eunhye Han
, Kyungmi Lee
, Sangho Lee
Korea Eundan, Korea
In this study, method validation for content analysis of the extracts of Aster glehni, a native plant of Ulleungdo, was conducted. The operating conditions were composed of LUNA C18 (5 μm, 250 ⅹ 4.6 mm, Phenomenex, Torrance, CA, USA) column, 1.0 mL/min for flow rate, and total 40 minutes for gradient elution parameter. In this process, a problem was posed that marker compound, 3,5-Dicaffeoylquinic acid(3,5-DCQA), peak was not separated completely from adjacent peak. To resolve this problem, resolution improvement of 3,5-DCQA peak was attempted by new analytical method. The resolution improvement research was conducted through changing analytical conditions such as column, flow rate, and gradient elution parameter. A column was changed to Kromasil 100-5-C18 (250 ⅹ 4.6 mm, 5 μm). Flow rate was changed to 0.8 mL/min. Gradient elution parameter was also revised to 26 minutes. A better resolution was confirmed with altered operating conditions. Consequently, method validation was conducted to verify the changed analytical method. The results of changing analytical method, it was obtained that resolution of 3,5-DCQA peak was higher than the standard resolution, 1.5. It was also confirmed that 3,5-DCQA peak was completely separated on the chromatogram without interference by adjacent peak. Furthermore, the results of method validation, system suitability, specificity, linearity, accuracy, and precision were meet the criteria.
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