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학술발표회초록보기

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  • 02월 19일 10시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제121회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Development of Plasmon Based Imaging Techniques to Break an Optical Diffraction Limit

등록일
2018년 2월 26일 10시 05분 11초
접수번호
6225
발표코드
ANAL1-1 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
목 15시 : 40분
발표형식
심포지엄
발표분야
Analytical Chemistry - Recent Advances in Analytical Chemistry I: Optical Sensor Platform Based Nanobio Materials
저자 및
공동저자
Kyujung Kim
Department of Optics and Mechatronics Engineering, Pusan National University, Korea
In this presentation, I introduce plasmon based nanoscale sampling methods to break a diffraction limit with nanostructures in total internal reflection fluorescence (TIRF) microscopy. Various fluorescent molecular targets, such as adenovirus, microtubules and proteins, were used to measure the imaging resolution for a broad application. Significant resolution improvements were achieved down to 70 nm imaging resolution without fundamental deviation from a conventional TIRF microscope. I compared those performances to an extraordinary transmission based axial imaging (EOT-AIM) technique using linear nanoaperture arrays. EOT-AIM uses linear arrays of nanoapertures, each of which samples target fluorescence up to a preset axial distance from surface, in combination with wide-field microscopy for acquisition of lateral images. A current design of nanoapertures provides EOT-AIM with axial super-resolution that is as small as 20 nm for a depth range of 500 nm. Experiments were performed for the measurement of axial distribution of intracellular cholera toxin subunit B molecules within mouse macrophage (RAW264.7) cells. The results were successfully confirmed with conventional confocal and total internal reflection fluorescence microscopy. Eventually, a merged plasmon based super resolution imaging platform was explored to analyze molecular events with nanoscale.

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