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  • 02월 19일 10시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제121회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Liquid chromatography/mass spectrometry (LC/MS) analysis of ganglioside analysis: the role of ammonium bicarbonate additive

2018년 2월 12일 14시 27분 08초
ANAL1.O-13 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
목 10시 : 31분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists I
저자 및
Jun young Park, Sangwon Cha1,*
Chemistry, Hankuk University of Foreign Studies, Korea
1Department of Chemistry, Hankuk University of Foreign Studies, Korea
Gangliosides are acidic glycosphingolipids which contain sugar chains with a variety of sialic acid residues. There are hundreds of variations in ganglioside structures based on compositions and structures of oligosaccharide head groups as well as compositions of ceramide cores. However, most LC/MS studies did not include separation of these structural isomers. Recently, we developed a simple and reliable LC method with a conventional C18 column that can effectively resolve ganglioside isomers such as structural isomers of disialogangliosides, designated to GD1a and GD1b, which are known to be major species in mammal brains. In this LC method, the key component was the salt additive, ammonium bicarbonate. Therefore, in this study, we focused on optimizing salt additive condition and trying to reveal a separation mechanism behind this phenomenon. Finally, we applied the LC/MS method with the optimized salt additive condition to the analysis of the total ganglioside extract from porcine brain and successfully analyzed individual isomers of di- and tri- gangliosides.