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학술발표회초록보기

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  • 02월 19일 10시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제121회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Large-area Graphene Films as Engaging Target Surfaces for Highly Reproducible Matrix-assisted Laser Desorption Ionization for Quantitative Mass Spectrometry

등록일
2018년 2월 13일 19시 04분 51초
접수번호
6514
발표코드
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발표시간
목 10시 : 39분
발표형식
구두발표
발표분야
Analytical Chemistry - Oral Presentation of Young Analytical Chemists I
저자 및
공동저자
YOONKYUNG CHOI, Sang Yun Han*
Department of Nano Chemistry, Gachon University Global Campus, Korea
Matrix-assisted laser desorption ionization (MALDI) mass spectrometry is a powerful means for characterization of large and complex molecules. However, due to the sweet spot issues that intrinsically arise from inhomogeneous matrix-analyte crystals utilized as MALDI samples, its reproducibility in ion generation and thus its applications for quantification have been rather limited. In this letter, we demonstrate a simple strategy to improve uniformity of matrix-analyte crystal spots, which was realized by adapting non-inert, interacting surfaces on purpose for the first time, i.e. large-area graphene films in this study, as target surfaces. The graphitic surfaces of graphene films may interact with excessive matrix molecules during the sample drying process, which induces apparently uniform crystallization of MALDI sample spots on the surfaces. Furthermore, high-resolution mass spectrometric imaging revealed that analytes embedded in the crystals formed on the graphene films were more evenly distributed over a MALDI sample spot than those commonly produced on the metal target plates, which as a result greatly suppresses appearance of sweet spots. In virtue of improved uniformity of MALDI sample spots, this method showed good linear responses of ion intensity to analyte concentrations (R2 > 0.99) as well as small fluctuations (s < 10%), which would be applicable for quantitative mass spectrometry using the MALDI method.

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