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  • 02월 19일 10시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제121회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Phospholipase A2-responsive UCNPs-loaded Phosphate Micelles for Prostate Cancer Cells Imaging

등록일
2018년 2월 7일 15시 17분 07초
접수번호
6616
발표코드
ANAL2.O-17 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 10시 : 25분
발표형식
구두발표
발표분야
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
공동저자
SHARIPOV MIRKOMIL, Salah Mahmoud Tawfik Ahmed, YONG-ILL LEE*
Department of Chemistry, Changwon National University, Korea
Rare-earth upconversion nanoparticles (UCNPs) have received the tremendous attention of researchers last decades due to their potential application as biological luminescent labels in bio-imaging application. However, it is still a challenge for researchers to obtain nontoxic, nanoscale, well dispersed and high selective nanoparticles. On the other hand, it has been reported that phospholipase A2(PLA-2) enzyme is abnormally overexpressed in several diseases such as pancreatitis or early stages of prostate cancer. Novel phosphate micelles from biocompatible compounds were synthesized and characterized for selective imaging of prostate cancer cells. Using fluorescence method, CMC value of the synthesized micelles was determined to be 0.638 mM. The activity of phospholipase A2 (PLA-2) enzyme toward the synthesized micelle was investigated and confirmed by LC-MS. This kind of surfactant can form UCNPs-loaded phosphate micelles formed from PLA-2 cleavable surfactant in order to deliver UCNPs directly to prostate tumor cells by reducing side effects on normal biological tissues. TEM results showed that micelles have size ranged from 80 nm to 150 nm; whereas size of UCNPs-loaded micelles ranges from 200nm to 350 nm. Cytotoxicity results confirmed the biocompatibility of micelles. Bio-imaging experiments conducted on KB and HeLa cell lines confirmed encapsulation of UCNPs in micelles thereby reducing the non-selective binding of UCNPs towards cells with low expression of PLA-2. Meanwhile, bio-imaging application on 22Rv1 prostate cancer cell line confirmed the selective release of UCNPs on the surface of the prostate cancer cells.reducing the non-selective binding of UCNPs towards cells with low expression of PLA-2. Meanwhile, bio-imaging application on 22Rv1 prostate cancer cell line confirmed the selective release of UCNPs on the surface of the prostate cancer cells.

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