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  • 02월 19일 10시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제121회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Determination of C18 -ceramide based on paper-based analytical device coupling with mass spectrometry

2018년 2월 7일 15시 21분 51초
ANAL2.O-18 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 10시 : 29분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
Shavkatjon Azizov, SHARIPOV MIRKOMIL, Huy B.T., Jae-Min Lim, YONG-ILL LEE*
Department of Chemistry, Changwon National University, Korea
Paper spray ionization has been developed as a direct, fast and low-cost sampling and ionization method for qualitative and quantitative mass spectrometric analysis of C18-ceramide in complex mixtures. Analyte ions are generated by applying a high voltage with a small volume of spray solvent onto a porous substrate. The sample can be preloaded onto paper or mixed into the spray solution. Wax-printed micro paper-based analytical device (µPAD) coated with paraffin was utilized as a high efficient and low-cost microfluidic device which can be combined in paper spray mass spectrometry (PS-MS). Until now, several methods have been introduced for C18-ceramide analysis. However, µPAD combined with PS-MS method has never used for C18-ceramide analysis. Mostly, high-performance liquid chromatography-mass spectrometry methodology is adapted for the determination of ceramide in human blood and serum samples but it needs sophisticated optimization of instrumentation and sample preparation. Our developed µPAD-paper spray mass spectrometry provides clear advantages over other methods due to reducing the experimental steps and fast operation process for identifying C18-ceramide.