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  • 02월 19일 10시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제121회 대한화학회 학술발표회, 총회 및 기기전시회 Development of a Multiple-color-based Three-dimensional Paper Sensor for Selective Detection of Foodborne Pathogens

2018년 2월 7일 17시 07분 51초
ANAL2.O-25 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
금 10시 : 53분
Analytical Chemistry - Oral Presentation of Young Analytical Chemists II
저자 및
Hyeok Jung Kim, Hyeran Noh1,*
Department of optometry, Seoul National University of Science & Technology, Korea
1Convergence Institute of Biomedical Engineering & Biomaterials, Seoul National University of Science & Technology, Korea
Sensing strategies for foodborne pathogens have been developing as rapidly analyzing, convenient and cost-effective sensors throughout the past decades. Here, we developed a three-dimensional paper sensor for five species of foodborne bacteria, Escherichia coli, Escherichia coli O:157, Salmonella spp., Staphylococcus and Listeria monocytogenes, with using the chromogenic substrates specific to the enzyme of each bacterium. This compact-sized sensor was fabricated with multi-layered filter paper which is patterned by wax-ink to form hydrophobized walls in the paper matrix. Each layer contains lysis, oxidizing and chromogenic substrate reagent for effective enzyme-substrate reaction. Each pathogen was qualitatively detected by observing the color developments of the bottom layer with naked eyes. Further quantitative analysis was realized by digitalizing the color changes. The color developments were detectable within 30 min after injecting 50μL of sample. When conducted with enrichment step (taking 12 hours or less), the sensor was able to sensitively detect as low concentration of the analytes as 101 cfu/ml. The three-dimensional paper sensor is potable, selective and easy-to-use analytic device for first screening detection and quantitative analysis of foodborne pathogen.