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제122회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Profiling of oxidized phospholipids in exosome and subcellular species of cells under oxidative stress by flow field-flow fractionation and nUHPLC-ESI-MS/MS

등록일
2018년 8월 17일 10시 42분 15초
접수번호
0306
발표코드
ANAL.P-332 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 19일 (금요일) 11:00~12:30
발표형식
포스터 분석구두발표
발표분야
Analytical Chemistry
저자 및
공동저자
JoonSeon Yang, Myeong Hee Moon*
Department of Chemistry, Yonsei University, Korea
Oxidative stress occurs when production of reactive oxygen species (ROS), which include oxygen derived radical species such as superoxide anion (O2∙-) and hydroxyl radical(∙OH) as well as hydrogen peroxide (H2O2), exceeds removal capacity of antioxidants. Moderate concentration of ROS is related to some beneficial physiological roles (e.g. signaling, host defense), but high levels of ROS not only induces cellular impairments by altering DNA, RNA, proteins, and lipids but also involves with a number of diseases like cardiovascular disease (CVD) or cancer. Exosomes are nano-sized extracellular vesicles secreted from cells. It has been reported that exosome transports some protective RNA against oxidative stress or transfers stress signals to recipient cells, when oxidative stress is given to cells. However, physiological roles or changes of lipids in exosome during oxidative stress conditions have not yet been studied. In this study, oxidative stress was induced to human embryonic kidney cell 293 (HEK293) by treating with H2O2 for 72 hours. Exosome and cellular organelles from control and oxidatively stressed conditions were size-sorted by flow field-flow fractionation first. Then, changes in lipid profiles in cell and exosome was conducted by nUHPLC-ESI-MS/MS.

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