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제122회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Separation of lipoproteins and exosome in plasma using frit-inlet asymmetrical flow field-flow fractionation with multi-angle light scattering

2018년 8월 17일 10시 43분 19초
ANAL.P-333 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 19일 (금요일) 11:00~12:30
포스터 분석구두발표
Analytical Chemistry
저자 및
Young Beom Kim, Myeong Hee Moon*
Department of Chemistry, Yonsei University, Korea
Lipoproteins transport hydrophobic molecules such as cholesterol in blood and are known to be associated with diseases such as arteriosclerosis. Lipoproteins are classified by high-density lipoprotein (HDL, 5~15 nm), low-density lipoprotein (LDL, 18~28 nm), very low-density lipoprotein (VLDL, 30~80 nm) according to its size and density. Exosomes are extracellular vesicles secreted by cells and their sizes are known as about 30~150 nm in diameter, which are larger than LDL but similar or slightly larger than VLDL. Exosomes are known to play an important role in the intercellular communication as a mediator of RNA, protein, and lipids, therefore, it can be expected to be utilized for disease prognosis and treatment. However, the separation of lipoproteins and exosomes from blood has not been studied extensively. Field-flow fractionation (FFF) is capable of separating sample components from nano to micron size. In this study, lipoproteins and exosomes from blood plasma samples were separated by frit-inlet asymmetrical flow field-flow fractionation (FI-AF4) using field programming and detected by UV spectrophotometer (UV) and multi-angle light scattering (MALS). After separation and fractionation by FI-AF4, lipoproteins and exosomes were confirmed by Western blot and transmission electron microscope (TEM).