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제122회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Optimal purification method for expression of human melanocortin-4 receptor

등록일
2018년 8월 17일 11시 48분 31초
접수번호
0318
발표코드
ANAL.P-336 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 19일 (금요일) 11:00~12:30
발표형식
포스터 분석구두발표
발표분야
Analytical Chemistry
저자 및
공동저자
Minseon Kim, Soyeon Jo1, Ji Sun Kim 2, Yongae Kim2,*
Department of chemistry, Hankuk University of Foreign Studies, Korea
1chemistry, Hankuk University of Foreign Studies, Korea
2Department of Chemistry, Hankuk University of Foreign Studies, Korea
The human transmembrane protein (hTMP) is the type of integral membrane proteins and exists as a signal transduction, intercellular communication, and ion channels, etc. Thus, it has various functions in the biological membrane. In order to demonstrate the function of the transmembrane proteins, the protein should be purified to identify the structure because function is related to structure closely. However, since the transmembrane protein is most composed of hydrophobic amino acids and is surrounded by the lipid, expression and purification of transmembrane protein are not easy. The transmembrane protein, which plays a biologically important role, is associated with the onset of many diseases. If a mutation occurs in human melanocortin-4 receptor (hMC4R), one of the transmembrane proteins, cause eating disorder and obesity. Asparagine-substituted mutants in aspartic acid, the 90th amino acid in the second transmembrane protein (TM2), were found in the patients with early onset obesity. It was thought that the loss of function was caused by structural changes of hMC4R due to the D90N mutation. Therefore, we examined the structural differences between wild-type hMC4R-TM2 (wt-hMC4R-TM2) and mutant hMC4R-TM2 (m-hMC4R-TM2) after expression and purification of two proteins. In the experimental process, SDS was used as a detergent when separating proteins using FPLC to separate them from impurities using hydrophobicity of target proteins. Afterwards, purified high yield target proteins were obtained by using SDS removal method, and the final structure was confirmed by various spectroscopic methods like MS, CD, solution NMR spectroscopy, and solid-state NMR spectroscopy.

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