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  • 09월 03일 23시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제122회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Double-Emulsion Nanoparticle Based on Biocompatible and Biodegradable L-Tyrosine Polyurethane for Efficient Gene Delivery

2018년 8월 20일 17시 04분 54초
POLY.P-4 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 18일 (목요일) 11:00~12:30
Polymer Chemistry
저자 및
Soo Yong Park, Ildoo Chung*
Department of Polymer Science and Engineering, Pusan National University, Korea

Gene therapy is a good alternative to drugs in chemotherapy due to drug resistance and toxicity and known to lower the probability of mutation of cells through gene carrier. Herein, gene carrier nanoparticles with minimal toxicity and high transfection efficiency were fabricated from biodegradable polymer (L-tyrosine polyurethane, LTU) which was presynthesized from desaminotyrosyl tyrosine hexyl ester (DTH), and polyethylene glycol (PEG) through double emulsion method and used to evaluate their potential biological activities molecualr controlled release and transfection studies. In order to evaluate cell adsorption and transfection of nanoparticles, two types of nanoparticles were prepared, the one from fluoresced using fluorescently labeled bovine serum albumin (FITC-BSA) to investigate cell adsorption, and the other from encapsulated with DNA-linear polyethylenimine (LPEI) complex to investigate the transfection efficiency in LX2 (human hepatic stellate cell), HepG2 (human liver cancer cell), MCF7 (human breast cancer cell). The morphology of these nanoparticles, which was confirmed both by microscope image and TEM, was a spherical shape with an average diameter of about 260 nm and 145 nm. The biodegradable nanoparticles showed the typical features of double emulsion. After 14 days, DNA in nanoparticles have been released from the LTU nanoparticles. Furthermore, a successful cellular uptake of LTU nanoparticles in hepatic stellate cells has been confirmed and high transfection efficiency also confirmed in LX2, HepG2 cells with check proper concentration of LPEI due to toxicity. These characteristics are ideal for gene therapy designed to transport and release a drug into the cytoplasm.