abs

학술발표회초록보기

초록문의 abstract@kcsnet.or.kr

결제문의 member@kcsnet.or.kr

현재 가능한 작업은 아래와 같습니다.
  • 09월 03일 23시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

대한화학회 제122회 학술발표회 및 총회 Development of a ganglioside profiling method by using a reversed phase liquid chromatography/mass spectrometry (RPLC/MS) platform

등록일
2018년 8월 21일 12시 32분 13초
접수번호
0565
발표코드
ANAL.P-344 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 19일 (금요일) 11:00~12:30
발표형식
포스터
발표분야
Analytical Chemistry
저자 및
공동저자
Jun young Park, TaeMin Park, Youngjoo Kal, Sangwon Cha*
Department of Chemistry, Hankuk University of Foreign Studies, Korea
Gangliosides are acidic glycosphingolipids which contain sugar chains with a variety of sialic acid residues. Gangliosides have several structural isomers of which glycan structures vary. However, most of reported reversed phase liquid chromatography/mass spectrometry (RPLC/MS) platforms could not separate these structural isomers. Recently, we have developed an RPLC method that could effectively separate the ganglioside isomers with a C18 column and an ammonium bicarbonate additive. In our previous study, the optimal salt condition which can give complete ganglioside isomer separation while maintaining high sensitivity was suggested. In addition, with an optimized RPLC/MS condition, we successfully profiled total gangliosides present in porcine brains with clear isomer differentiation. In this study, we further optimized our method by evaluating the limits of detection (LODs) of the major gangliosides, peak capacity, peak resolution, and so on.

상단으로