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대한화학회 제122회 학술발표회 및 총회 Hypersensitive Cancer Immunosensor Based on One-Shot Dual Signal Detection on Nano-patterned Biochip

등록일
2018년 8월 21일 15시 20분 55초
접수번호
0615
발표코드
ANAL.P-345 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 19일 (금요일) 11:00~12:30
발표형식
포스터
발표분야
Analytical Chemistry
저자 및
공동저자
Seungah Lee, Seong Ho Kang*
Department of Applied Chemistry, Kyung Hee University, Korea
Three-dimensional enhanced dark-field super-resolution microscopy (3D EDF-SRM) based on hypersensitive one-shot dual signal detection was developed for immunoassay of TNF-a as a cancer biomarker. The 3D EDF-SRM is equipped with a dual view and a digital single-reflex (DSLR) camera, enabling dual signal detection which was both quantifiable and qualitatively. The standard TNF-a was quantitatively analyzed by measuring the scattering signals of 80 nm silver nanotag on an array chip with 100 nm gold spot. The localization precision in nanotag on the gold spot narrowed to <10 nm after applying the least-cubic algorithm. The developed hypersensitive immunosensor exhibited a detection limit of zeptomolar level (S/N = 3) with a wide dynamic detection range of zM-pM. In addition, a recovery greater than 98% was achieved by spiking standard TNF-a into human serum samples.

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