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  • 08월 30일 20시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

The cibacron blue-binding proteins in Rice and E. coli

등록일
2006년 9월 11일 11시 17분 49초
접수번호
1294
발표코드
28P280포 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
금 <발표Ⅱ>
발표형식
포스터
발표분야
생명화학
저자 및
공동저자
김미옥, 정제훈
전남대학교 화학과,
The immobilized Cibacron Blue F3GA has been widely applied for purifying proteins requiring ATP, NAD+, or NADP+ as a cofactor. The soluble proteins from rice and E. coli were applied to a Cibacron blue-Sepharose column and the strongly bound proteins were isolated by a buffer containing 1M sodium chloride. Over 121 rice proteins were resolved by two-dimensional gel electrophoresis and 97 proteins were identified by MALDI-TOF mass spectrometer. Over 139 E. coli proteins were resolved and 119 proteins were identified. Some of the identified proteins might bind to Cibacron Blue indirectly through protein-protein interactions with Cibacron Blue-binding proteins. Identified were also 33 hypothetical proteins with no known functions. Those 33 proteins were target proteins for studying whether they require ATP or NADH(NADPH) as cofactor or not. The method described in this study is very effective for probing hypothetical proteins.

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