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  • 09월 03일 23시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제122회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Preparation and Physicochemical Characterization of Magnetic Agarose Beads for Protein Purification

2018년 8월 23일 16시 36분 59초
POLY.P-27 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 18일 (목요일) 11:00~12:30
Polymer Chemistry
저자 및
So-Yeon Kim, Kyoung-Mahn Kim1, Hasoo Seong*
Therapeutics & Biotechnology Division, Korea Research Institute of Chemical Technology, Korea
1Research Strategy Division, Korea Research Institute of Chemical Technology, Korea
Agarose beads have been studied for efficient separation and purification of tagged specific proteins. In this study, agarose beads having magnetic iron oxide particles were prepared by using emulsion droplet gelation method and their physicochemical characteristics were studied. Black Fe3O4 particles were prepared by co-precipitation of Fe(II) and Fe(III) ions with an alkaline solution. Agarose aqueous droplets having Fe3O4 particles were formed in a water-in-oil emulsion prepared by emulsification of the hot agarose aqueous solution in an organic solvent such as cyclohexane. Gelation of agarose by cooling of the droplets gave magnetic agarose beads. An average size of the beads prepared at fairly optimized conditions was 100 micrometers. The Fe3O4 contents in agarose beads were determined by an inductively coupled plasma analysis. Crystalline peaks of Fe3O4 particles and changes of the peaks of magnetic agarose beads were identified by X-ray diffraction analyses. To strengthen magnetic agarose beads, the agarose was crosslinked with epichlorohydrin. Physicochemical properties of the crosslinked magnetic beads were characterized by swelling ratio measurement and FT-IR, TGA, DSC analyses etc.