Abstract : Anthocyanin is an antioxidant, and is of various types. In this study, the type and the content of anthocyanin in red wine were determined. For comparisons, five commercial red wines were chosen, one French wine and four Argentinian wines. First, a wine sample was injected into an asymmetrical flow field-flow fractionation (AsFlFFF) to separate the components in the sample. Then the anthocyanin fraction from AsFlFFF fractogram was collected, and injected into HPLC (C18, 2.7 µm×100 mm) for quantitative analysis after a sample preparation procedure shown in Scheme 1 below, which includes evaporation and hydrolysis. Significant differences were found among the tested wine samples of various sources in both the type and the content of anthocyanin.
AsFlFFF provided an useful tool for separation of anthocyanin from red wine, and thus for HPLC analysis of anthocyanin, while the size-exclusion chromatography (SEC) suffer from interactions between the wine components and the stationary phase.
References :  A. Vernhet, S. Dubascoux, B. Cabane, H. Fulcrand, E. Dubreucq and C. Poncet-Legrand, Anal Bioanal Chem 2011, 401, 1559–1569.