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  • 09월 03일 23시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제122회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Nanobead-based sensitive immunosensor using amphiphilic functional copolymer

등록일
2018년 8월 30일 12시 51분 28초
접수번호
2036
발표코드
MAT.P-466 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 18일 (목요일) 11:00~12:30
발표형식
포스터
발표분야
Material Chemistry
저자 및
공동저자
Daekyung Sung
Center for Convergence Bioceramic Materials, Korea Institute of Ceramic Engineering and Technology, Korea
We developed nanobead multilayer-based sensitive immunoassays using amphiphilic functional copolymer. We developed an amphiphilic copolymer comprising benzyl, polyethylene glycol, and reactive ester moieties. Polystyrene nanobeads were reacted with the polymer-coated COC surface and further assembled into multilayers that increased the overall surface area available for attaching capture antibodies. Finally, a sandwich immunoassay was carried out using rabbit IgG as a target analyte and rhodamine-labeled anti-rabbit IgG as a probe. Compared with a nanobead-free, polymer-coated COC surface, the nanobead multilayer-based immunoassay exhibited ~4-fold higher fluorescence intensity. In addition, our nanobead-based assay system exhibited a wide dynamic range of detection (0.1 to 1000 ng/mL) and high specificity for rabbit IgG, indicating the potential value of the proposed immunoassay system in plastic-based portable biochip applications.

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