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학술발표회초록보기

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제122회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Application of immunosensor using QD Immobilized Silica Nanoparticles for signal amplification

등록일
2018년 8월 30일 12시 52분 46초
접수번호
2039
발표코드
INOR.P-176 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
발표시간
10월 19일 (금요일) 11:00~12:30
발표형식
포스터
발표분야
Inorganic Chemistry
저자 및
공동저자
Daekyung Sung
Center for Convergence Bioceramic Materials, Korea Institute of Ceramic Engineering and Technology, Korea
QD functionalized silica nanoparticles was developed for application of immunosensor using nonbiofouling, pre-activated polymers coated substrates for cancer marker detection. In this work, we synthesized random copolymers having three important groups: anchoring sites, serving to attach the COC substrate; a PEG component, which acts as a repellent of non-specific biomolecules; and an NHS ester group for conjugation of biomolecules. For sandwich immunoassays, anti-α-fetoprotein (Anti-AFP) as capture antibody was covalently immobilized on the substrate through the polymer layers. Furthermore, we prepared anti-AFP/QD-coated silica nanospheres conjugates for signal amplification. Sandwich-type immunoassays were carried out on the polymer coated COC surfaces using AFP (liver cancer marker). Using the optimized assay, the red fluorescence response to AFP was linear over a range from 0.1 to 100 ng/ml.

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