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  • 09월 03일 23시 이후 : 초록수정 불가능, 일정확인 및 검색만 가능

제122회 대한화학회 학술발표회, 총회 및 기기전시회 안내 Naked-eye sensitive detection of tuberculosis specific antigen based on copper-gold enhancement immunoassay

2018년 8월 30일 14시 30분 35초
MEDI.P-324 이곳을 클릭하시면 발표코드에 대한 설명을 보실 수 있습니다.
10월 18일 (목요일) 11:00~12:30
Medicinal Chemistry
저자 및
Le Minh Tu Phan, Tae Jung Park*
Department of Chemistry, Chung-Ang University, Korea
Conventional diagnosis procedures for identifying biomarker such as microbiological test, immunological test, biochemical processes required long time duration, labour-intensive instruments, exorbitant costs and caused false results. Silver nanoshell enhancement on the gold nanoparticles (AuNPs) surface is usually performed in immunoassay platform to intensify the colorimetric signal for the detection of biomarkers but lack of selectivity and sensitivity limit its application as a biodetection label. Herein, a straightforward and highly specific dot-blot immunoassay was successfully developed to detect Mycobacterium tuberculosis secretory antigen, CFP-10, based on copper nanoshell formation on the AuNPs surface. The reduction capacity of sodium L-ascorbate to the Cu-PEI complex can form the copper nanoshell on the AuNPs surface, results in the double amplification of the optical signals than that of conventional silver nanoshell. For biomarker diagnostics, this strategy was applied to detect CFP-10 tuberculosis antigen, and the magnified signals can be strongly detectable with naked-eye or a smartphone camera with the detection limit as low as 8 pg/mL. A simple copper enhancing bioassay can identify the CFP-10 antigen in clinical urine sample with high sensitivity, specificity, low cost as well as minimization of complicated procedures. This facile and sensitive bioassay exhibits great application potential for highly reliable point-of-care detection of other biomarkers in biomedical field or life science